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Fakultät Bio- und Chemieingenieurwesen

Cosolvent and Substrate Influence in Enzymatic Catalyzed Reactions for Production of Fine Chemicals

07/01/2020 - 07/31/2024

Enzyme-catalyzed reactions are used in industry, but the equilibrium and kinetics of such reactions are still often limited. The oxidation reaction of the formate anion catalyzed by formate dehydrogenase is investigated in this project as a model system for a pressure-induced switchable reaction systems towards production of either the fine chemical NADH or for CO2 conversion.


In this project, different parameters of the reactions such as enzyme, pressure, pH, temperature, or the addition of co-solvents will be studied to improve the reaction rates and, therefore, the operability and space-time yield of the reactions. Besides, ePC-SAFT will be used for the prediction of thermodynamic and kinetic parameters that describe the reaction, which leads to a reduction in the experimental effort [3]. Finally, it is planned to optimize the up- and downstream processing of a potential process involving CO2 as a substrate.


[1] Straathof, A. J. J.; Panke, S.; Schmid, A:
"The Production of Fine Chemicals by Biotransformations"
Current Opinion in Biotechnology. 2002, 13 (6), 548–556.
[2] Woodley, J. M.:
"New Frontiers in Biocatalysis for Sustainable Synthesis"
Current Opinion in Green and Sustainable Chemistry. February 1, 2020, pp 22–26
[3] C. Held, T. Reschke, S. Mohammad, A. Luza, G. Sadowski:
"ePC-SAFT revised"
Chemical Engineering Research and Design. 2014, vol. 92, pp. 2884-2897